Construct: shRNA TRCN0000044666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019842.2-1195s1c1
DNA Barcode:
CCAACCTCATTCAGTGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNQ5 (56479)
Gene Description:
potassium voltage-gated channel subfamily Q member 5
Transcript:
RefSeq NM_019842.2 (NON-CURRENT)
Match location:
Position 1195 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACCTCATTCAGTGTGTTT
Hairpin Sequence:
5'-CCGG-CCAACCTCATTCAGTGTGTTT-CTCGAG-AAACACACTGAATGAGGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACCTCATTCAGTGTGTTTCTCGAGAAACACACTGAATGAGGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACCTCATTCAGTGTGTTTCTCGAGAAACACACTGAATGAGGTTGG-3'

Other clones with same target sequence:

(none)