Construct: shRNA TRCN0000044682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022054.2-760s1c1
DNA Barcode:
CGTCGTTTCCACCATAGGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK13 (56659)
Gene Description:
potassium two pore domain channel subfamily K member 13
Transcript:
RefSeq NM_022054.2 (NON-CURRENT)
Match location:
Position 760 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCGTTTCCACCATAGGGTT
Hairpin Sequence:
5'-CCGG-CGTCGTTTCCACCATAGGGTT-CTCGAG-AACCCTATGGTGGAAACGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCGTTTCCACCATAGGGTTCTCGAGAACCCTATGGTGGAAACGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCGTTTCCACCATAGGGTTCTCGAGAACCCTATGGTGGAAACGACG-3'

Other clones with same target sequence:

(none)