Construct: shRNA TRCN0000044706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004174.1-2260s1c1
DNA Barcode:
AGGAATTGACAACCCTGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC9A3 (6550)
Gene Description:
solute carrier family 9 member A3
Transcript:
RefSeq NM_004174.1 (NON-CURRENT)
Match location:
Position 2260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAATTGACAACCCTGTGTT
Hairpin Sequence:
5'-CCGG-AGGAATTGACAACCCTGTGTT-CTCGAG-AACACAGGGTTGTCAATTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAATTGACAACCCTGTGTTCTCGAGAACACAGGGTTGTCAATTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAATTGACAACCCTGTGTTCTCGAGAACACAGGGTTGTCAATTCCT-3'

Other clones with same target sequence:

(none)