Construct: shRNA TRCN0000044730

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020122.2-547s1c1
DNA Barcode:
GCAGTTCTACTGGTGGACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCMF1 (56888)
Gene Description:
potassium channel modulatory factor 1
Transcript:
RefSeq NM_020122.2 (NON-CURRENT)
Match location:
Position 547 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044730)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTCTACTGGTGGACTTT
Hairpin Sequence:
5'-CCGG-GCAGTTCTACTGGTGGACTTT-CTCGAG-AAAGTCCACCAGTAGAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTCTACTGGTGGACTTTCTCGAGAAAGTCCACCAGTAGAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTCTACTGGTGGACTTTCTCGAGAAAGTCCACCAGTAGAACTGC-3'

Other clones with same target sequence:

  1. TRCN0000288980