Construct: shRNA TRCN0000044775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152333.1-440s1c1
DNA Barcode:
CTCAACCAGTTCCTGGCAGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A29 (123096)
Gene Description:
solute carrier family 25 member 29
Transcript:
RefSeq NM_152333.1 (NON-CURRENT)
Match location:
Position 440 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAACCAGTTCCTGGCAGGT
Hairpin Sequence:
5'-CCGG-CTCAACCAGTTCCTGGCAGGT-CTCGAG-ACCTGCCAGGAACTGGTTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAACCAGTTCCTGGCAGGTCTCGAGACCTGCCAGGAACTGGTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAACCAGTTCCTGGCAGGTCTCGAGACCTGCCAGGAACTGGTTGAG-3'

Other clones with same target sequence:

(none)