Construct: shRNA TRCN0000044787
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004975.2-2579s1c1
- DNA Barcode:
- CCCTAAGTTCTTAAGGCAGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KCNB1 (3745)
- Gene Description:
- potassium voltage-gated channel subfamily B member 1
- Transcript:
- RefSeq NM_004975.2 (NON-CURRENT)
- Match location:
- Position 2579 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000044787)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCTAAGTTCTTAAGGCAGAA
- Hairpin Sequence:
-
5'-CCGG-CCCTAAGTTCTTAAGGCAGAA-CTCGAG-TTCTGCCTTAAGAACTTAGGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCTAAGTTCTTAAGGCAGAACTCGAGTTCTGCCTTAAGAACTTAGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCTAAGTTCTTAAGGCAGAACTCGAGTTCTGCCTTAAGAACTTAGGG-3'
Other clones with same target sequence:
(none)
