Construct: shRNA TRCN0000044837

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173508.1-711s1c1
DNA Barcode:
CGTCTGCAAGTCCACAGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC35F3 (148641)
Gene Description:
solute carrier family 35 member F3
Transcript:
RefSeq NM_173508.1 (NON-CURRENT)
Match location:
Position 711 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044837)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGCAAGTCCACAGAGAA
Hairpin Sequence:
5'-CCGG-CGTCTGCAAGTCCACAGAGAA-CTCGAG-TTCTCTGTGGACTTGCAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGCAAGTCCACAGAGAACTCGAGTTCTCTGTGGACTTGCAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGCAAGTCCACAGAGAACTCGAGTTCTCTGTGGACTTGCAGACG-3'

Other clones with same target sequence:

(none)