Construct: shRNA TRCN0000044861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003636.2-514s1c1
DNA Barcode:
CCTCGTCATCACCACCAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNAB2 (8514)
Gene Description:
potassium voltage-gated channel subfamily A regulatory beta subunit 2
Transcript:
RefSeq NM_003636.2 (NON-CURRENT)
Match location:
Position 514 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGTCATCACCACCAAGAT
Hairpin Sequence:
5'-CCGG-CCTCGTCATCACCACCAAGAT-CTCGAG-ATCTTGGTGGTGATGACGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGTCATCACCACCAAGATCTCGAGATCTTGGTGGTGATGACGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGTCATCACCACCAAGATCTCGAGATCTTGGTGGTGATGACGAGG-3'

Other clones with same target sequence:

(none)