Construct: shRNA TRCN0000044904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004366.2-432s1c1
DNA Barcode:
CATCACTTTCTCAGCCGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLCN2 (1181)
Gene Description:
chloride voltage-gated channel 2
Transcript:
RefSeq NM_004366.2 (NON-CURRENT)
Match location:
Position 432 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCACTTTCTCAGCCGGATT
Hairpin Sequence:
5'-CCGG-CATCACTTTCTCAGCCGGATT-CTCGAG-AATCCGGCTGAGAAAGTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCACTTTCTCAGCCGGATTCTCGAGAATCCGGCTGAGAAAGTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCACTTTCTCAGCCGGATTCTCGAGAATCCGGCTGAGAAAGTGATG-3'

Other clones with same target sequence:

(none)