Construct: shRNA TRCN0000044913

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003740.2-621s1c1
DNA Barcode:
CGTCATTACCACCATTGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK5 (8645)
Gene Description:
potassium two pore domain channel subfamily K member 5
Transcript:
RefSeq NM_003740.2 (NON-CURRENT)
Match location:
Position 621 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044913)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCATTACCACCATTGGATA
Hairpin Sequence:
5'-CCGG-CGTCATTACCACCATTGGATA-CTCGAG-TATCCAATGGTGGTAATGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCATTACCACCATTGGATACTCGAGTATCCAATGGTGGTAATGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCATTACCACCATTGGATACTCGAGTATCCAATGGTGGTAATGACG-3'

Other clones with same target sequence:

(none)