Construct: shRNA TRCN0000044931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001479.1-526s1c1
DNA Barcode:
ACCTGGCACAACTGGTTACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC35E4 (339665)
Gene Description:
solute carrier family 35 member E4
Transcript:
RefSeq NM_001001479.1 (NON-CURRENT)
Match location:
Position 526 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTGGCACAACTGGTTACTA
Hairpin Sequence:
5'-CCGG-ACCTGGCACAACTGGTTACTA-CTCGAG-TAGTAACCAGTTGTGCCAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTGGCACAACTGGTTACTACTCGAGTAGTAACCAGTTGTGCCAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTGGCACAACTGGTTACTACTCGAGTAGTAACCAGTTGTGCCAGGT-3'

Other clones with same target sequence:

(none)