Construct: shRNA TRCN0000045020

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004980.3-2417s1c1
DNA Barcode:
CCAGCAATGTTGTCAAGGTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCND3 (3752)
Gene Description:
potassium voltage-gated channel subfamily D member 3
Transcript:
RefSeq NM_004980.3 (NON-CURRENT)
Match location:
Position 2417 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045020)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCAATGTTGTCAAGGTCT
Hairpin Sequence:
5'-CCGG-CCAGCAATGTTGTCAAGGTCT-CTCGAG-AGACCTTGACAACATTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCAATGTTGTCAAGGTCTCTCGAGAGACCTTGACAACATTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCAATGTTGTCAAGGTCTCTCGAGAGACCTTGACAACATTGCTGG-3'

Other clones with same target sequence:

(none)