Construct: shRNA TRCN0000045155

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005832.3-968s1c1
DNA Barcode:
CTCCCTACTATGTGAGAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNMB2 (10242)
Gene Description:
potassium calcium-activated channel subfamily M regulatory beta subunit 2
Transcript:
RefSeq NM_005832.3 (NON-CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045155)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCCTACTATGTGAGAGGAT
Hairpin Sequence:
5'-CCGG-CTCCCTACTATGTGAGAGGAT-CTCGAG-ATCCTCTCACATAGTAGGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCCTACTATGTGAGAGGATCTCGAGATCCTCTCACATAGTAGGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCCTACTATGTGAGAGGATCTCGAGATCCTCTCACATAGTAGGGAG-3'

Other clones with same target sequence:

(none)