Construct: shRNA TRCN0000045166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018398.1-1818s1c1
DNA Barcode:
CGAGGGAATGTAACCATCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNA2D3 (55799)
Gene Description:
calcium voltage-gated channel auxiliary subunit alpha2delta 3
Transcript:
RefSeq NM_018398.1 (NON-CURRENT)
Match location:
Position 1818 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGGAATGTAACCATCGAA
Hairpin Sequence:
5'-CCGG-CGAGGGAATGTAACCATCGAA-CTCGAG-TTCGATGGTTACATTCCCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGGAATGTAACCATCGAACTCGAGTTCGATGGTTACATTCCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGGAATGTAACCATCGAACTCGAGTTCGATGGTTACATTCCCTCG-3'

Other clones with same target sequence:

(none)