Construct: shRNA TRCN0000045444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001875.2-2365s1c1
DNA Barcode:
CCAGAAATTAAGAACGTCGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPS1 (1373)
Gene Description:
carbamoyl-phosphate synthase 1
Transcript:
RefSeq NM_001875.2 (NON-CURRENT)
Match location:
Position 2365 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAATTAAGAACGTCGTA
Hairpin Sequence:
5'-CCGG-CCAGAAATTAAGAACGTCGTA-CTCGAG-TACGACGTTCTTAATTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAATTAAGAACGTCGTACTCGAGTACGACGTTCTTAATTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAATTAAGAACGTCGTACTCGAGTACGACGTTCTTAATTTCTGG-3'

Other clones with same target sequence:

(none)