Construct: shRNA TRCN0000045465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014918.3-2339s1c1
DNA Barcode:
CCCAGTTTAACAATGAATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHSY1 (22856)
Gene Description:
chondroitin sulfate synthase 1
Transcript:
RefSeq NM_014918.3 (NON-CURRENT)
Match location:
Position 2339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTTTAACAATGAATCTT
Hairpin Sequence:
5'-CCGG-CCCAGTTTAACAATGAATCTT-CTCGAG-AAGATTCATTGTTAAACTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTTTAACAATGAATCTTCTCGAGAAGATTCATTGTTAAACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTTTAACAATGAATCTTCTCGAGAAGATTCATTGTTAAACTGGG-3'

Other clones with same target sequence:

(none)