Construct: shRNA TRCN0000045592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052946.2-1146s1c1
DNA Barcode:
GCATACTCATAGCTATGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOSTRIN (115677)
Gene Description:
nitric oxide synthase trafficking
Transcript:
RefSeq NM_052946.2 (NON-CURRENT)
Match location:
Position 1146 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATACTCATAGCTATGTGAA
Hairpin Sequence:
5'-CCGG-GCATACTCATAGCTATGTGAA-CTCGAG-TTCACATAGCTATGAGTATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATACTCATAGCTATGTGAACTCGAGTTCACATAGCTATGAGTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATACTCATAGCTATGTGAACTCGAGTTCACATAGCTATGAGTATGC-3'

Other clones with same target sequence:

(none)