Construct: shRNA TRCN0000045626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014697.1-1197s1c1
DNA Barcode:
GCTCCCTTCTTCTTCCTCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOS1AP (9722)
Gene Description:
nitric oxide synthase 1 adaptor protein
Transcript:
RefSeq NM_014697.1 (NON-CURRENT)
Match location:
Position 1197 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCCTTCTTCTTCCTCGAA
Hairpin Sequence:
5'-CCGG-GCTCCCTTCTTCTTCCTCGAA-CTCGAG-TTCGAGGAAGAAGAAGGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCCTTCTTCTTCCTCGAACTCGAGTTCGAGGAAGAAGAAGGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCCTTCTTCTTCCTCGAACTCGAGTTCGAGGAAGAAGAAGGGAGC-3'

Other clones with same target sequence:

(none)