Construct: shRNA TRCN0000045845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002130.3-741s1c1
DNA Barcode:
CCTGATATGCTATCTGAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMGCS1 (3157)
Gene Description:
3-hydroxy-3-methylglutaryl-CoA synthase 1
Transcript:
RefSeq NM_002130.3 (NON-CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATATGCTATCTGAATAT
Hairpin Sequence:
5'-CCGG-CCTGATATGCTATCTGAATAT-CTCGAG-ATATTCAGATAGCATATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATATGCTATCTGAATATCTCGAGATATTCAGATAGCATATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATATGCTATCTGAATATCTCGAGATATTCAGATAGCATATCAGG-3'

Other clones with same target sequence:

(none)