Construct: shRNA TRCN0000045851

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006295.1-3294s1c1
DNA Barcode:
GTCTACTTGGAGTGCCTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VARS1 (7407)
Gene Description:
valyl-tRNA synthetase 1
Transcript:
RefSeq NM_006295.1 (NON-CURRENT)
Match location:
Position 3294 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045851)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTACTTGGAGTGCCTGAAA
Hairpin Sequence:
5'-CCGG-GTCTACTTGGAGTGCCTGAAA-CTCGAG-TTTCAGGCACTCCAAGTAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTACTTGGAGTGCCTGAAACTCGAGTTTCAGGCACTCCAAGTAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTACTTGGAGTGCCTGAAACTCGAGTTTCAGGCACTCCAAGTAGAC-3'

Other clones with same target sequence:

  1. TRCN0000286184