Construct: shRNA TRCN0000045861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005518.1-271s1c1
DNA Barcode:
GAGAAGTATAACAATGTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMGCS2 (3158)
Gene Description:
3-hydroxy-3-methylglutaryl-CoA synthase 2
Transcript:
RefSeq NM_005518.1 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGTATAACAATGTGGAA
Hairpin Sequence:
5'-CCGG-GAGAAGTATAACAATGTGGAA-CTCGAG-TTCCACATTGTTATACTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGTATAACAATGTGGAACTCGAGTTCCACATTGTTATACTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGTATAACAATGTGGAACTCGAGTTCCACATTGTTATACTTCTC-3'

Other clones with same target sequence:

(none)