Construct: shRNA TRCN0000045863

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022745.1-1530s1c1
DNA Barcode:
CCATCAGGTTAAAGACTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATPAF1 (64756)
Gene Description:
ATP synthase mitochondrial F1 complex assembly factor 1
Transcript:
RefSeq NM_022745.1 (NON-CURRENT)
Match location:
Position 1530 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045863)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAGGTTAAAGACTTCAT
Hairpin Sequence:
5'-CCGG-CCATCAGGTTAAAGACTTCAT-CTCGAG-ATGAAGTCTTTAACCTGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAGGTTAAAGACTTCATCTCGAGATGAAGTCTTTAACCTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAGGTTAAAGACTTCATCTCGAGATGAAGTCTTTAACCTGATGG-3'

Other clones with same target sequence:

(none)