Construct: shRNA TRCN0000045871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145691.2-486s1c1
DNA Barcode:
GTGCAACACATCATTGGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATPAF2 (91647)
Gene Description:
ATP synthase mitochondrial F1 complex assembly factor 2
Transcript:
RefSeq NM_145691.2 (NON-CURRENT)
Match location:
Position 486 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCAACACATCATTGGACAA
Hairpin Sequence:
5'-CCGG-GTGCAACACATCATTGGACAA-CTCGAG-TTGTCCAATGATGTGTTGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCAACACATCATTGGACAACTCGAGTTGTCCAATGATGTGTTGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCAACACATCATTGGACAACTCGAGTTGTCCAATGATGTGTTGCAC-3'

Other clones with same target sequence:

  1. TRCN0000290109