Construct: shRNA TRCN0000045877

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001673.2-646s1c1
DNA Barcode:
GCTGTATGTTCAGAAGCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASNS (440)
Gene Description:
asparagine synthetase (glutamine-hydrolyzing)
Transcript:
RefSeq NM_001673.2 (NON-CURRENT)
Match location:
Position 646 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045877)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTATGTTCAGAAGCTAAA
Hairpin Sequence:
5'-CCGG-GCTGTATGTTCAGAAGCTAAA-CTCGAG-TTTAGCTTCTGAACATACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTATGTTCAGAAGCTAAACTCGAGTTTAGCTTCTGAACATACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTATGTTCAGAAGCTAAACTCGAGTTTAGCTTCTGAACATACAGC-3'

Other clones with same target sequence:

  1. TRCN0000290113