Construct: shRNA TRCN0000045892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015340.3-2561s1c1
DNA Barcode:
CCCTCATGTAACCTCAGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LARS2 (23395)
Gene Description:
leucyl-tRNA synthetase 2, mitochondrial
Transcript:
RefSeq NM_015340.3 (NON-CURRENT)
Match location:
Position 2561 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCATGTAACCTCAGAGAT
Hairpin Sequence:
5'-CCGG-CCCTCATGTAACCTCAGAGAT-CTCGAG-ATCTCTGAGGTTACATGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCATGTAACCTCAGAGATCTCGAGATCTCTGAGGTTACATGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCATGTAACCTCAGAGATCTCGAGATCTCTGAGGTTACATGAGGG-3'

Other clones with same target sequence:

(none)