Construct: shRNA TRCN0000045931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004957.2-361s1c1
DNA Barcode:
TCACGGAATGTATCCTCCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FPGS (2356)
Gene Description:
folylpolyglutamate synthase
Transcript:
RefSeq NM_004957.2 (NON-CURRENT)
Match location:
Position 361 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACGGAATGTATCCTCCGAA
Hairpin Sequence:
5'-CCGG-TCACGGAATGTATCCTCCGAA-CTCGAG-TTCGGAGGATACATTCCGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACGGAATGTATCCTCCGAACTCGAGTTCGGAGGATACATTCCGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACGGAATGTATCCTCCGAACTCGAGTTCGGAGGATACATTCCGTGA-3'

Other clones with same target sequence:

(none)