Construct: shRNA TRCN0000046010

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000240.2-1680s1c1
DNA Barcode:
GCCTGCTGAAGATCATTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAOA (4128)
Gene Description:
monoamine oxidase A
Transcript:
RefSeq NM_000240.2 (NON-CURRENT)
Match location:
Position 1680 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046010)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGCTGAAGATCATTGGAT
Hairpin Sequence:
5'-CCGG-GCCTGCTGAAGATCATTGGAT-CTCGAG-ATCCAATGATCTTCAGCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGCTGAAGATCATTGGATCTCGAGATCCAATGATCTTCAGCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGCTGAAGATCATTGGATCTCGAGATCCAATGATCTTCAGCAGGC-3'

Other clones with same target sequence:

(none)