Construct: shRNA TRCN0000046128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017545.2-763s1c1
DNA Barcode:
GCTGTTAAACATGGCTTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HAO1 (54363)
Gene Description:
hydroxyacid oxidase 1
Transcript:
RefSeq NM_017545.2 (NON-CURRENT)
Match location:
Position 763 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTAAACATGGCTTGAAT
Hairpin Sequence:
5'-CCGG-GCTGTTAAACATGGCTTGAAT-CTCGAG-ATTCAAGCCATGTTTAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTAAACATGGCTTGAATCTCGAGATTCAAGCCATGTTTAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTAAACATGGCTTGAATCTCGAGATTCAAGCCATGTTTAACAGC-3'

Other clones with same target sequence:

(none)