Construct: shRNA TRCN0000046290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001861.2-230s1c1
DNA Barcode:
AGCTCATGAAAGTGTTGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COX4I1 (1327)
Gene Description:
cytochrome c oxidase subunit 4I1
Transcript:
RefSeq NM_001861.2 (NON-CURRENT)
Match location:
Position 230 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTCATGAAAGTGTTGTGAA
Hairpin Sequence:
5'-CCGG-AGCTCATGAAAGTGTTGTGAA-CTCGAG-TTCACAACACTTTCATGAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTCATGAAAGTGTTGTGAACTCGAGTTCACAACACTTTCATGAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTCATGAAAGTGTTGTGAACTCGAGTTCACAACACTTTCATGAGCT-3'

Other clones with same target sequence:

(none)