Construct: shRNA TRCN0000046403

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005989.1-778s1c1
DNA Barcode:
CCTTTGTTAAAGGATGCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKR1D1 (6718)
Gene Description:
aldo-keto reductase family 1 member D1
Transcript:
RefSeq NM_005989.1 (NON-CURRENT)
Match location:
Position 778 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046403)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGTTAAAGGATGCACTT
Hairpin Sequence:
5'-CCGG-CCTTTGTTAAAGGATGCACTT-CTCGAG-AAGTGCATCCTTTAACAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGTTAAAGGATGCACTTCTCGAGAAGTGCATCCTTTAACAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGTTAAAGGATGCACTTCTCGAGAAGTGCATCCTTTAACAAAGG-3'

Other clones with same target sequence:

(none)