Construct: shRNA TRCN0000046412

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001628.2-444s1c1
DNA Barcode:
CCATTGGATGAGTCGGGCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKR1B1 (231)
Gene Description:
aldo-keto reductase family 1 member B
Transcript:
RefSeq NM_001628.2 (NON-CURRENT)
Match location:
Position 444 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046412)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGGATGAGTCGGGCAAT
Hairpin Sequence:
5'-CCGG-CCATTGGATGAGTCGGGCAAT-CTCGAG-ATTGCCCGACTCATCCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGGATGAGTCGGGCAATCTCGAGATTGCCCGACTCATCCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGGATGAGTCGGGCAATCTCGAGATTGCCCGACTCATCCAATGG-3'

Other clones with same target sequence:

  1. TRCN0000288738