Construct: shRNA TRCN0000046478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003366.1-606s1c1
DNA Barcode:
GCCTTGGCTAATCCCTTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UQCRC2 (7385)
Gene Description:
ubiquinol-cytochrome c reductase core protein 2
Transcript:
RefSeq NM_003366.1 (NON-CURRENT)
Match location:
Position 606 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGGCTAATCCCTTGTAT
Hairpin Sequence:
5'-CCGG-GCCTTGGCTAATCCCTTGTAT-CTCGAG-ATACAAGGGATTAGCCAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGGCTAATCCCTTGTATCTCGAGATACAAGGGATTAGCCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGGCTAATCCCTTGTATCTCGAGATACAAGGGATTAGCCAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000286329