Construct: shRNA TRCN0000046638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022119.2-636s1c1
DNA Barcode:
GTTCCTATCATCGACTCGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRSS22 (64063)
Gene Description:
serine protease 22
Transcript:
RefSeq NM_022119.2 (NON-CURRENT)
Match location:
Position 636 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCCTATCATCGACTCGGAA
Hairpin Sequence:
5'-CCGG-GTTCCTATCATCGACTCGGAA-CTCGAG-TTCCGAGTCGATGATAGGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCCTATCATCGACTCGGAACTCGAGTTCCGAGTCGATGATAGGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCCTATCATCGACTCGGAACTCGAGTTCCGAGTCGATGATAGGAAC-3'

Other clones with same target sequence:

(none)