Construct: shRNA TRCN0000046846

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144662.1-360s1c1
DNA Barcode:
GTAGTAATAAACAGAGCCCGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMA8 (143471)
Gene Description:
proteasome 20S subunit alpha 8
Transcript:
RefSeq NM_144662.1 (NON-CURRENT)
Match location:
Position 360 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046846)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGTAATAAACAGAGCCCGT
Hairpin Sequence:
5'-CCGG-GTAGTAATAAACAGAGCCCGT-CTCGAG-ACGGGCTCTGTTTATTACTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGTAATAAACAGAGCCCGTCTCGAGACGGGCTCTGTTTATTACTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGTAATAAACAGAGCCCGTCTCGAGACGGGCTCTGTTTATTACTAC-3'

Other clones with same target sequence:

(none)