Construct: shRNA TRCN0000046866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139054.2-2687s1c1
DNA Barcode:
CCAGCATAAAGCAAATGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAMTS18 (170692)
Gene Description:
ADAM metallopeptidase with thrombospondin type 1 motif 18
Transcript:
RefSeq NM_139054.2 (NON-CURRENT)
Match location:
Position 2687 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000046866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCATAAAGCAAATGAATA
Hairpin Sequence:
5'-CCGG-CCAGCATAAAGCAAATGAATA-CTCGAG-TATTCATTTGCTTTATGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCATAAAGCAAATGAATACTCGAGTATTCATTTGCTTTATGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCATAAAGCAAATGAATACTCGAGTATTCATTTGCTTTATGCTGG-3'

Other clones with same target sequence:

(none)