Construct: shRNA TRCN0000047003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020361.2-424s1c1
DNA Barcode:
CCCAGCAGTATCTCCTATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPA6 (57094)
Gene Description:
carboxypeptidase A6
Transcript:
RefSeq NM_020361.2 (NON-CURRENT)
Match location:
Position 424 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCAGTATCTCCTATGTA
Hairpin Sequence:
5'-CCGG-CCCAGCAGTATCTCCTATGTA-CTCGAG-TACATAGGAGATACTGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCAGTATCTCCTATGTACTCGAGTACATAGGAGATACTGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCAGTATCTCCTATGTACTCGAGTACATAGGAGATACTGCTGGG-3'

Other clones with same target sequence:

(none)