Construct: shRNA TRCN0000047037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003474.2-2237s1c1
DNA Barcode:
GCCTGAATCGTCAATGTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAM12 (8038)
Gene Description:
ADAM metallopeptidase domain 12
Transcript:
RefSeq NM_003474.2 (NON-CURRENT)
Match location:
Position 2237 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAATCGTCAATGTCAAA
Hairpin Sequence:
5'-CCGG-GCCTGAATCGTCAATGTCAAA-CTCGAG-TTTGACATTGACGATTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAATCGTCAATGTCAAACTCGAGTTTGACATTGACGATTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAATCGTCAATGTCAAACTCGAGTTTGACATTGACGATTCAGGC-3'

Other clones with same target sequence:

(none)