Construct: shRNA TRCN0000047125

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020742.2-924s1c1
DNA Barcode:
CCCACGGAAGATGATATTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NLGN4X (57502)
Gene Description:
neuroligin 4 X-linked
Transcript:
RefSeq NM_020742.2 (NON-CURRENT)
Match location:
Position 924 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047125)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACGGAAGATGATATTCAT
Hairpin Sequence:
5'-CCGG-CCCACGGAAGATGATATTCAT-CTCGAG-ATGAATATCATCTTCCGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACGGAAGATGATATTCATCTCGAGATGAATATCATCTTCCGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACGGAAGATGATATTCATCTCGAGATGAATATCATCTTCCGTGGG-3'

Other clones with same target sequence:

(none)