Construct: shRNA TRCN0000047131

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152627.1-1648s1c1
DNA Barcode:
CCACCTAAGAAGCTAATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC14C (168448)
Gene Description:
cell division cycle 14C
Transcript:
RefSeq NM_152627.1 (NON-CURRENT)
Match location:
Position 1648 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047131)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTAAGAAGCTAATCAAT
Hairpin Sequence:
5'-CCGG-CCACCTAAGAAGCTAATCAAT-CTCGAG-ATTGATTAGCTTCTTAGGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTAAGAAGCTAATCAATCTCGAGATTGATTAGCTTCTTAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTAAGAAGCTAATCAATCTCGAGATTGATTAGCTTCTTAGGTGG-3'

Other clones with same target sequence:

(none)