Construct: shRNA TRCN0000047133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000601.3-1512s2c1
DNA Barcode:
CCCGTAATATCTTGTGCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HGF (3082)
Gene Description:
hepatocyte growth factor
Transcript:
RefSeq NM_000601.3 (NON-CURRENT)
Match location:
Position 1512 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTAATATCTTGTGCCAAA
Hairpin Sequence:
5'-CCGG-CCCGTAATATCTTGTGCCAAA-CTCGAG-TTTGGCACAAGATATTACGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTAATATCTTGTGCCAAACTCGAGTTTGGCACAAGATATTACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTAATATCTTGTGCCAAACTCGAGTTTGGCACAAGATATTACGGG-3'

Other clones with same target sequence:

  1. TRCN0000003306