Construct: shRNA TRCN0000047157
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_024989.2-2153s1c1
- DNA Barcode:
- CCTCTGAACTTCCTAAAGATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PGAP1 (80055)
- Gene Description:
- post-GPI attachment to proteins inositol deacylase 1
- Transcript:
- RefSeq NM_024989.2 (NON-CURRENT)
- Match location:
- Position 2153 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000047157)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTCTGAACTTCCTAAAGATA
- Hairpin Sequence:
-
5'-CCGG-CCTCTGAACTTCCTAAAGATA-CTCGAG-TATCTTTAGGAAGTTCAGAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTCTGAACTTCCTAAAGATACTCGAGTATCTTTAGGAAGTTCAGAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTCTGAACTTCCTAAAGATACTCGAGTATCTTTAGGAAGTTCAGAGG-3'
Other clones with same target sequence:
(none)
