Construct: shRNA TRCN0000047158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006125.1-3151s1c1
DNA Barcode:
CCCTTATTTGATGTTTCTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP6 (395)
Gene Description:
Rho GTPase activating protein 6
Transcript:
RefSeq NM_006125.1 (NON-CURRENT)
Match location:
Position 3151 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTATTTGATGTTTCTGTA
Hairpin Sequence:
5'-CCGG-CCCTTATTTGATGTTTCTGTA-CTCGAG-TACAGAAACATCAAATAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTATTTGATGTTTCTGTACTCGAGTACAGAAACATCAAATAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTATTTGATGTTTCTGTACTCGAGTACAGAAACATCAAATAAGGG-3'

Other clones with same target sequence:

(none)