Construct: shRNA TRCN0000047236
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_014504.1-1422s1c1
- DNA Barcode:
- GTTCAAGACATCGTTGAGAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RABGEF1 (27342)
- Gene Description:
- RAB guanine nucleotide exchange factor 1
- Transcript:
- RefSeq NM_014504.1 (NON-CURRENT)
- Match location:
- Position 1422 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000047236)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTTCAAGACATCGTTGAGAAA
- Hairpin Sequence:
-
5'-CCGG-GTTCAAGACATCGTTGAGAAA-CTCGAG-TTTCTCAACGATGTCTTGAAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTTCAAGACATCGTTGAGAAACTCGAGTTTCTCAACGATGTCTTGAACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTTCAAGACATCGTTGAGAAACTCGAGTTTCTCAACGATGTCTTGAAC-3'
Other clones with same target sequence:
(none)
