Construct: shRNA TRCN0000047244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001715.1-584s1c1
DNA Barcode:
CCAAGCACCATTCACATCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FARP1 (10160)
Gene Description:
FERM, ARH/RhoGEF and pleckstrin domain protein 1
Transcript:
RefSeq NM_001001715.1 (NON-CURRENT)
Match location:
Position 584 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCACCATTCACATCTAA
Hairpin Sequence:
5'-CCGG-CCAAGCACCATTCACATCTAA-CTCGAG-TTAGATGTGAATGGTGCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCACCATTCACATCTAACTCGAGTTAGATGTGAATGGTGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCACCATTCACATCTAACTCGAGTTAGATGTGAATGGTGCTTGG-3'

Other clones with same target sequence:

  1. TRCN0000291823