Construct: shRNA TRCN0000047265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018287.4-852s1c1
DNA Barcode:
CCCAGGAACAATCTTGTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP12 (94134)
Gene Description:
Rho GTPase activating protein 12
Transcript:
RefSeq NM_018287.4 (NON-CURRENT)
Match location:
Position 852 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGAACAATCTTGTGATT
Hairpin Sequence:
5'-CCGG-CCCAGGAACAATCTTGTGATT-CTCGAG-AATCACAAGATTGTTCCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGAACAATCTTGTGATTCTCGAGAATCACAAGATTGTTCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGAACAATCTTGTGATTCTCGAGAATCACAAGATTGTTCCTGGG-3'

Other clones with same target sequence:

(none)