Construct: shRNA TRCN0000047266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018287.4-1539s1c1
DNA Barcode:
GCACCATTGTATTGGACACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP12 (94134)
Gene Description:
Rho GTPase activating protein 12
Transcript:
RefSeq NM_018287.4 (NON-CURRENT)
Match location:
Position 1539 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCATTGTATTGGACACTA
Hairpin Sequence:
5'-CCGG-GCACCATTGTATTGGACACTA-CTCGAG-TAGTGTCCAATACAATGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCATTGTATTGGACACTACTCGAGTAGTGTCCAATACAATGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCATTGTATTGGACACTACTCGAGTAGTGTCCAATACAATGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000291761