Construct: shRNA TRCN0000047300

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004308.2-552s1c1
DNA Barcode:
GCATCCAACCATGTTCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP1 (392)
Gene Description:
Rho GTPase activating protein 1
Transcript:
RefSeq NM_004308.2 (NON-CURRENT)
Match location:
Position 552 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047300)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCAACCATGTTCATCAA
Hairpin Sequence:
5'-CCGG-GCATCCAACCATGTTCATCAA-CTCGAG-TTGATGAACATGGTTGGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCAACCATGTTCATCAACTCGAGTTGATGAACATGGTTGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCAACCATGTTCATCAACTCGAGTTGATGAACATGGTTGGATGC-3'

Other clones with same target sequence:

  1. TRCN0000291688