Construct: shRNA TRCN0000047333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014570.3-169s1c1
DNA Barcode:
GCCCACTAACAAGGTGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARFGAP3 (26286)
Gene Description:
ADP ribosylation factor GTPase activating protein 3
Transcript:
RefSeq NM_014570.3 (NON-CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCACTAACAAGGTGTGTTT
Hairpin Sequence:
5'-CCGG-GCCCACTAACAAGGTGTGTTT-CTCGAG-AAACACACCTTGTTAGTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCACTAACAAGGTGTGTTTCTCGAGAAACACACCTTGTTAGTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCACTAACAAGGTGTGTTTCTCGAGAAACACACCTTGTTAGTGGGC-3'

Other clones with same target sequence:

(none)