Construct: shRNA TRCN0000047352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014990.1-2271s1c1
DNA Barcode:
CCTGGATCAAAGCAAACCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALGAPA1 (253959)
Gene Description:
Ral GTPase activating protein catalytic alpha subunit 1
Transcript:
RefSeq NM_014990.1 (NON-CURRENT)
Match location:
Position 2271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGATCAAAGCAAACCTAA
Hairpin Sequence:
5'-CCGG-CCTGGATCAAAGCAAACCTAA-CTCGAG-TTAGGTTTGCTTTGATCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGATCAAAGCAAACCTAACTCGAGTTAGGTTTGCTTTGATCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGATCAAAGCAAACCTAACTCGAGTTAGGTTTGCTTTGATCCAGG-3'

Other clones with same target sequence:

(none)