Construct: shRNA TRCN0000047407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012294.2-105s1c1
DNA Barcode:
CCTCATTTGGAGAGGAAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAPGEF5 (9771)
Gene Description:
Rap guanine nucleotide exchange factor 5
Transcript:
RefSeq NM_012294.2 (NON-CURRENT)
Match location:
Position 105 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATTTGGAGAGGAAAGAT
Hairpin Sequence:
5'-CCGG-CCTCATTTGGAGAGGAAAGAT-CTCGAG-ATCTTTCCTCTCCAAATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATTTGGAGAGGAAAGATCTCGAGATCTTTCCTCTCCAAATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATTTGGAGAGGAAAGATCTCGAGATCTTTCCTCTCCAAATGAGG-3'

Other clones with same target sequence:

(none)